Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.
Identifieur interne : 001D00 ( Main/Exploration ); précédent : 001C99; suivant : 001D01Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.
Auteurs : S H Wang [République populaire de Chine] ; Y. Li [République populaire de Chine] ; Z Q Li [République populaire de Chine] ; L. Chang [République populaire de Chine] ; L. Li [République populaire de Chine]Source :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Marqueurs biologiques.
- génétique : Salicaceae.
- méthodes : Technique RAPD.
- physiologie : Salicaceae.
- Amorces ADN, Marqueurs génétiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Biomarkers.
- chemical : DNA Primers, Genetic Markers.
- genetics : Salicaceae.
- methods : Random Amplified Polymorphic DNA Technique.
- physiology : Salicaceae.
Abstract
Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify. A total of 342 primer combinations were screened and 2770 bands were examined. Only me14/em8 could amplify a specific fragment (210 base pairs) in all female but none in male plants. We analyzed this fragment using GenBank and found that the sequence similarity was 80% to the Populus trichocarpa clone POP006-H09 (sequence ID: gb|AC212923.1|) and that of the deduced amino acid sequence was 73% to the integrase of Mendicago truncatula (sequence ID: gb|ABD28291.1|) and 71% to the predicted retrotransposon integrase-like protein 1-like in Cicer arietinum (sequence ID: ref|XP 004515460.1|) (NCBI database through December 17, 2013). This fragment was converted into a stable and simple sequence-characterized amplified region marker approximately 200 base pairs in length. This marker can be utilized for early sexual identification in I. polycarpa, which will facilitate future breeding programs.
DOI: 10.4238/2015.March.20.11
PubMed: 25867347
Affiliations:
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Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Li, Z Q" sort="Li, Z Q" uniqKey="Li Z" first="Z Q" last="Li">Z Q Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China lizhouqi@nwsuaf.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Chang, L" sort="Chang, L" uniqKey="Chang L" first="L" last="Chang">L. 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Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Li, Z Q" sort="Li, Z Q" uniqKey="Li Z" first="Z Q" last="Li">Z Q Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China lizhouqi@nwsuaf.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Chang, L" sort="Chang, L" uniqKey="Chang L" first="L" last="Chang">L. Chang</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author><author><name sortKey="Li, L" sort="Li, L" uniqKey="Li L" first="L" last="Li">L. Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomarkers (analysis)</term><term>DNA Primers (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Random Amplified Polymorphic DNA Technique (methods)</term><term>Salicaceae (genetics)</term><term>Salicaceae (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Marqueurs biologiques (analyse)</term><term>Marqueurs génétiques (MeSH)</term><term>Salicaceae (génétique)</term><term>Salicaceae (physiologie)</term><term>Technique RAPD (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Salicaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Salicaceae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Technique RAPD</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Salicaceae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Salicaceae</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Marqueurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify. A total of 342 primer combinations were screened and 2770 bands were examined. Only me14/em8 could amplify a specific fragment (210 base pairs) in all female but none in male plants. We analyzed this fragment using GenBank and found that the sequence similarity was 80% to the Populus trichocarpa clone POP006-H09 (sequence ID: gb|AC212923.1|) and that of the deduced amino acid sequence was 73% to the integrase of Mendicago truncatula (sequence ID: gb|ABD28291.1|) and 71% to the predicted retrotransposon integrase-like protein 1-like in Cicer arietinum (sequence ID: ref|XP 004515460.1|) (NCBI database through December 17, 2013). This fragment was converted into a stable and simple sequence-characterized amplified region marker approximately 200 base pairs in length. This marker can be utilized for early sexual identification in I. polycarpa, which will facilitate future breeding programs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25867347</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>08</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1676-5680</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Mar</Month><Day>20</Day></PubDate></JournalIssue><Title>Genetics and molecular research : GMR</Title><ISOAbbreviation>Genet Mol Res</ISOAbbreviation></Journal><ArticleTitle>Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.</ArticleTitle><Pagination><MedlinePgn>2015-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4238/2015.March.20.11</ELocationID><Abstract><AbstractText>Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify. A total of 342 primer combinations were screened and 2770 bands were examined. Only me14/em8 could amplify a specific fragment (210 base pairs) in all female but none in male plants. We analyzed this fragment using GenBank and found that the sequence similarity was 80% to the Populus trichocarpa clone POP006-H09 (sequence ID: gb|AC212923.1|) and that of the deduced amino acid sequence was 73% to the integrase of Mendicago truncatula (sequence ID: gb|ABD28291.1|) and 71% to the predicted retrotransposon integrase-like protein 1-like in Cicer arietinum (sequence ID: ref|XP 004515460.1|) (NCBI database through December 17, 2013). This fragment was converted into a stable and simple sequence-characterized amplified region marker approximately 200 base pairs in length. This marker can be utilized for early sexual identification in I. polycarpa, which will facilitate future breeding programs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>S H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Z Q</ForeName><Initials>ZQ</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China lizhouqi@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>03</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Genet Mol Res</MedlineTA><NlmUniqueID>101169387</NlmUniqueID><ISSNLinking>1676-5680</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019105" MajorTopicYN="N">Random Amplified Polymorphic DNA Technique</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031308" MajorTopicYN="N">Salicaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25867347</ArticleId><ArticleId IdType="pii">gmr4440</ArticleId><ArticleId IdType="doi">10.4238/2015.March.20.11</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, S H" sort="Wang, S H" uniqKey="Wang S" first="S H" last="Wang">S H Wang</name></noRegion><name sortKey="Chang, L" sort="Chang, L" uniqKey="Chang L" first="L" last="Chang">L. Chang</name><name sortKey="Li, L" sort="Li, L" uniqKey="Li L" first="L" last="Li">L. Li</name><name sortKey="Li, Y" sort="Li, Y" uniqKey="Li Y" first="Y" last="Li">Y. Li</name><name sortKey="Li, Z Q" sort="Li, Z Q" uniqKey="Li Z" first="Z Q" last="Li">Z Q Li</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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